Culture of mononuclear phagocytes on a teflon surface to prevent adherence

Culture of Mononuclear Phagocytes on a Teflon Surface to Prevent Adherence*

Brief Definitive Report CULTURE OF MONONUCLEAR PHAGOCYTES ON A TEFLON SURFACE TO PREVENT ADHERENCE*

Mononuclear phagocytes are usually cultured on a glass or plastic surface to which these cells readily become attached and spread. Both glass and plastic have, however, the disadvantage that it is very difficult to recover mononuclear phagocytes without injuring the cells. The present paper describes a method for culturing on a Teflon membrane to which cells do not attach and from which they ca...

Characteristics of long-term cultures of proliferating, mononuclear phagocytes from bone marrow.

The characteristics of murine bone marrow mononuclear phagocytes in long-term cultures with embryonic fibroblast-conditioned medium were studied to determine the stage of development and state of activation of these cells. Two liquid culture systems were used: for studies on the morphology, cytochemistry, and functional characteristics at the cellular level, the cells were cultured adherent to ...

Paradoxical priming effects of IL-10 on cytokine production.

IL-10 is a well-known immunosuppressive and/or anti-inflammatory cytokine. However, we report in vitro experimental studies in which IL-10 primed leukocytes and led to an enhanced production of tumor necrosis factor (TNF) upon further stimulation by lipopolysaccharide (LPS). Monocytes and peripheral blood mononuclear cells (PBMC) prepared from whole blood maintained for 20 h at 37 degrees C in ...

The influence of culture conditions and serum lipids on interleukin-1 production by human monocytes.

Reliable assessment of IL-1 production by human monocytes is critically dependent on the methods for isolation and culture of these cells. In the present study, the quality of pipettes and the preparation of Ficoll-Isopaque appear to be crucial for IL-1 production from both LPS-stimulated and unstimulated monocytes. Different brands and lots of polystyrene culture wells give rise to great varia...